FastQCFastQC Report
Sat 22 Apr 2017
SRR1976948_2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename SRR1976948_2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1000000
Filtered Sequences 0
Sequence length 251
%GC 45

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 1100375 6.571517 23.379585 240-247
CCCCC 661030 4.7139206 11.045358 240-247
AAAAA 1622005 4.304051 7.25235 240-247
TTTTT 1340165 3.8110087 4.51884 8
CGGGG 505080 3.1253042 8.077499 240-247
CCGGG 462745 2.9667506 5.8500285 240-247
CCCGG 439495 2.9194467 5.1772265 240-247
GGCGG 438430 2.712891 5.5525045 240-247
CCCCG 384040 2.6432018 5.0517774 240-247
GGGGC 419900 2.5982323 6.298634 240-247
GGGCG 357040 2.209271 5.268765 240-247
GCGGG 347730 2.151663 5.131194 240-247
Produced by FastQC (version 0.10.1)